Western blot analysis of SBDS in extracts of various cell lines. Samples were incubated with SBDS Polyclonal antibody (Product # PA5-96457) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 15s.
Immunocytochemistry-Immunofluorescence analysis of SBDS was performed in MCF-7 cells using SBDS Polyclonal Antibody (Product # PA5-96457).
Supplier Page from Thermo Fisher Scientific for SBDS Antibody